Protein PhysicoChemical Properties

Accession Number: pdtdbt00004

Target sequence:
        10         20         30         40         50         60         70         80         90        100 
MTPPHNYLAV IKVVGIGGGG VNAVNRMIEQ GLKGVEFIAI NTDAQALLMS DADVKLDVGR DSTRGLGAGA DPEVGRKAAE DAKDEIEELL RGADMVFVTA 
       110        120        130        140        150        160        170        180        190        200 
GEGGGTGTGG APVVASIARK LGALTVGVVT RPFSFEGKRR SNQAENGIAA LRESCDTLIV IPNDRLLQMG DAAVSLMDAF RSADEVLLNG VQGITDLITT 
       210        220        230        240        250        260        270        280        290        300 
PGLINVDFAD VKGIMSGAGT ALMGIGSARG EGRSLKAAEI AINSPLLEAS MEGAQGVLMS IAGGSDLGLF EINEAASLVQ DAAHPDANII FGTVIDDSLG 
       310        320        330        340        350        360        370 
DEVRVTVIAA GFDVSGPGRK PVMGETGGAH RIESAKAGKL TSTLFEPVDA VSVPLHTNGA TLSIGGDDDD VDVPPFMRR
Number of amino acids: 379

Molecular weight: 38755.8

Theoretical pI: 4.55

Amino acid composition: 
Ala (A)  46      12.1%
Arg (R)  19       5.0%
Asn (N)  13       3.4%
Asp (D)  30       7.9%
Cys (C)   1       0.3%
Gln (Q)   7       1.8%
Glu (E)  22       5.8%
Gly (G)  52      13.7%
His (H)   4       1.1%
Ile (I)  25       6.6%
Leu (L)  32       8.4%
Lys (K)  12       3.2%
Met (M)  12       3.2%
Phe (F)  11       2.9%
Pro (P)  15       4.0%
Ser (S)  22       5.8%
Thr (T)  20       5.3%
Trp (W)   0       0.0%
Tyr (Y)   1       0.3%
Val (V)  35       9.2%
Pyl (O)   0       0.0%
Sec (U)   0       0.0%

Asx (B)   0       0.0%
Glx (Z)   0       0.0%
Xaa (X)   0       0.0%
Total number of negatively charged residues (Asp + Glu): 52

Total number of positively charged residues (Arg + Lys): 31

Atomic composition: 
Carbon      C         1678
Hydrogen    H         2744
Nitrogen    N          476
Oxygen      O          547
Sulfur      S           13
Formula: C1678H2744N476O547S13

Total number of atoms: 5458

Extinction coefficients: 
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     1490
Abs 0.1% (=1 g/l)   0.038, assuming all pairs of Cys residues form cystines

Ext. coefficient     1490
Abs 0.1% (=1 g/l)   0.038, assuming all Cys residues are reduced
Estimated half-life: 
The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hour (mammalian reticulocytes, in vitro).
                            >20 hour (yeast, in vivo).
                            >10 hour (Escherichia coli, in vivo).
Instability index:

The instability index (II) is computed to be 29.27
This classifies the protein as stable.

Aliphatic index: 97.57

Aromaticity (Y+W+F): 3.2%

Grand average of hydropathicity (GRAVY): 0.119

Kyte & Doolittle Hydrophobicity Plot:

Score: -2.878 to 2.544
Position: 1 to 379
Window Size: 9 Hydrophobicity Plot