Protein PhysicoChemical Properties

Accession Number: pdtdbt00005

Target sequence:
        10         20         30         40         50         60         70         80         90 
SMKGIEKEVN VYKSEDSLGL TITDNGVGYA FIKRIKDGGV IDSVKTICVG DHIESINGEN IVGWRHYDVA KKLKELKKEE LFTMKLIEPK KSSEA
Number of amino acids: 95

Molecular weight: 10614.2

Theoretical pI: 6.70

Amino acid composition: 
Ala (A)   3       3.2%
Arg (R)   2       2.1%
Asn (N)   4       4.2%
Asp (D)   6       6.3%
Cys (C)   1       1.1%
Gln (Q)   0       0.0%
Glu (E)  10      10.5%
Gly (G)   9       9.5%
His (H)   2       2.1%
Ile (I)  10      10.5%
Leu (L)   6       6.3%
Lys (K)  14      14.7%
Met (M)   2       2.1%
Phe (F)   2       2.1%
Pro (P)   1       1.1%
Ser (S)   7       7.4%
Thr (T)   4       4.2%
Trp (W)   1       1.1%
Tyr (Y)   3       3.2%
Val (V)   8       8.4%
Pyl (O)   0       0.0%
Sec (U)   0       0.0%

Asx (B)   0       0.0%
Glx (Z)   0       0.0%
Xaa (X)   0       0.0%
Total number of negatively charged residues (Asp + Glu): 16

Total number of positively charged residues (Arg + Lys): 16

Atomic composition: 
Carbon      C          472
Hydrogen    H          770
Nitrogen    N          124
Oxygen      O          146
Sulfur      S            3
Formula: C472H770N124O146S3

Total number of atoms: 1515

Extinction coefficients: 
Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.939, assuming all pairs of Cys residues form cystines

Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.939, assuming all Cys residues are reduced
Estimated half-life: 
The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hour (mammalian reticulocytes, in vitro).
                            >20 hour (yeast, in vivo).
                            >10 hour (Escherichia coli, in vivo).
Instability index:

The instability index (II) is computed to be 52.87
This classifies the protein as unstable.

Aliphatic index: 93.26

Aromaticity (Y+W+F): 6.3%

Grand average of hydropathicity (GRAVY): -0.418

Kyte & Doolittle Hydrophobicity Plot:

Score: -2.1 to 1.222
Position: 1 to 95
Window Size: 9 Hydrophobicity Plot