Riboflavin (Vitamin B₂) is part of the Vitamin B group. It is the central component of the cofactors FAD and FMN and as such required for a variety of flavoprotein enzyme reactions including activation of other vitamins. It was formerly known as Vitamin G. The name "Riboflavin" comes from "Ribose" (the sugar whose reduced form, Ribitol, forms part of its structure) and "Flavin", the ring-moiety which imparts the yellow color to the oxidized molecule (from Latin flavus, "yellow"). The reduced form, which occurs in metabolism along with the oxidized form, is colorless.

Riboflavin is a B vitamin. It can be found in certain foods such as milk, meat, eggs, nuts, enriched flour, and green vegetables. Riboflavin is frequently used in combination with other 'B' Vitamins in Vitamin B complex products. Vitamin B complex generally includes Vitamin B₁ (Thiamine), Vitamin B₂ (Riboflavin), Vitamin B₃ (Niacin/Niacinamide), Vitamin B₅ (Pantothenic acid), Vitamin B₆ (Pyridoxine), Vitamin B₁₂ (Cyanocobalamin), and Folic acid. However, some products do not contain all of these ingredients and some may include others, such as Biotin, Para-aminobenzoic acid (PABA), Choline bitartrate, and Inositol.

Riboflavin is used for preventing low levels of Riboflavin (riboflavin deficiency), cervical cancer, and migraine headaches. It is also used for treating riboflavin deficiency, acne, muscle cramps, burning feet syndrome, carpal tunnel syndrome, and blood disorders such as congenital methemoglobinemia and red blood cell aplasia. Some people use riboflavin for eye conditions including eye fatigue, cataracts, and glaucoma.

Other uses include increasing energy levels; boosting immune system function; maintaining healthy hair, skin, mucous membranes, and nails; slowing aging; boosting athletic performance; promoting healthy reproductive function; canker sores; memory loss, including Alzheimer's disease; ulcers; burns; alcoholism; liver disease; sickle cell anemia; and treating lactic acidosis brought on by treatment with a class of AIDS medications called NRTI drugs.

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