Protein PhysicoChemical Properties

Accession Number: pdtdbt00003

Target sequence:
        10         20         30         40         50         60         70         80         90        100 
SKKNSLALSL TADQMVSALL DAEPPILYSE YDPTRPFSEA SMMGLLTNLA DRELVHMINW AKRVPGFVDL TLHDQVHLLE CAWLEILMIG LVWRSMEHPG 
       110        120        130        140        150        160        170        180        190        200 
KLLFAPNLLL DRNQGKCVEG MVEIFDMLLA TSSRFRMMNL QGEEFVCLKS IILLNSGVYT FLSSTLKSLE EKDHIHRVLD KITDTLIHLM AKAGLTLQQQ 
       210        220        230        240        250 
HQRLAQLLLI LSHIRHMSNK GMEHLYSMKC KNVVPLYDLL LEMLDAHRLH APTS
Number of amino acids: 254

Molecular weight: 28939.0

Theoretical pI: 6.34

Amino acid composition: 
Ala (A)  15       5.9%
Arg (R)  11       4.3%
Asn (N)   9       3.5%
Asp (D)  13       5.1%
Cys (C)   4       1.6%
Gln (Q)   9       3.5%
Glu (E)  15       5.9%
Gly (G)  10       3.9%
His (H)  13       5.1%
Ile (I)  12       4.7%
Leu (L)  48      18.9%
Lys (K)  13       5.1%
Met (M)  15       5.9%
Phe (F)   7       2.8%
Pro (P)   9       3.5%
Ser (S)  19       7.5%
Thr (T)  11       4.3%
Trp (W)   3       1.2%
Tyr (Y)   5       2.0%
Val (V)  13       5.1%
Pyl (O)   0       0.0%
Sec (U)   0       0.0%

Asx (B)   0       0.0%
Glx (Z)   0       0.0%
Xaa (X)   0       0.0%
Total number of negatively charged residues (Asp + Glu): 28

Total number of positively charged residues (Arg + Lys): 24

Atomic composition: 
Carbon      C         1294
Hydrogen    H         2087
Nitrogen    N          347
Oxygen      O          364
Sulfur      S           19
Formula: C1294H2087N347O364S19

Total number of atoms: 4111

Extinction coefficients: 
Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24200
Abs 0.1% (=1 g/l)   0.836, assuming all pairs of Cys residues form cystines

Ext. coefficient    23950
Abs 0.1% (=1 g/l)   0.828, assuming all Cys residues are reduced
Estimated half-life: 
The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hour (mammalian reticulocytes, in vitro).
                            >20 hour (yeast, in vivo).
                            >10 hour (Escherichia coli, in vivo).
Instability index:

The instability index (II) is computed to be 38.87
This classifies the protein as stable.

Aliphatic index: 112.87

Aromaticity (Y+W+F): 5.9%

Grand average of hydropathicity (GRAVY): 0.090

Kyte & Doolittle Hydrophobicity Plot:

Score: -2.178 to 2.511
Position: 1 to 254
Window Size: 9 Hydrophobicity Plot